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HOXB6-WG-MSCV
(Plasmid #20993)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    W->G mutation in YPWM interaction motif for PBX proteins.
  • Entrez Gene
    HOXB6 (a.k.a. HOX2, HOX2B, HU-2, Hox-2.2)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer MSCV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein does not bind PBX

Has IRES-GFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB6-WG-MSCV was a gift from Corey Largman (Addgene plasmid # 20993 ; http://n2t.net/addgene:20993 ; RRID:Addgene_20993)

  • For your References section:

    HOXB6 protein is bound to CREB-binding protein and represses globin expression in a DNA binding-dependent, PBX interaction-independent process. Shen W, Chrobak D, Krishnan K, Lawrence HJ, Largman C. J Biol Chem. 2004 Sep 17. 279(38):39895-904. 10.1074/jbc.M404132200 PubMed 15269212
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