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Addgene

LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA
(Plasmid #209922)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209922 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiCRISPRv2puro
  • Backbone manufacturer
    https://www.addgene.org/52961/
  • Total vector size (bp) 13013
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    A gRNA targeting the dog CDH1 gene.
  • gRNA/shRNA sequence
    CDH1

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA was a gift from Michiyuki Matsuda (Addgene plasmid # 209922 ; http://n2t.net/addgene:209922 ; RRID:Addgene_209922)

  • For your References section:

    Low-affinity ligands of the epidermal growth factor receptor are long-range signal transmitters in collective cell migration of epithelial cells. Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K. Cell Rep. 2024 Nov 14;43(11):114986. doi: 10.1016/j.celrep.2024.114986. 10.1016/j.celrep.2024.114986 PubMed 39546398
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