HOXB6-NA-MSCV
(Plasmid
#20992)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6500
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB6
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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MutationN51N
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Entrez GeneHOXB6 (a.k.a. HOX2, HOX2B, HU-2, Hox-2.2)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Product of N->A mutation is a DNA-binding mutant.
Has IRES-GF
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXB6-NA-MSCV was a gift from Corey Largman (Addgene plasmid # 20992 ; http://n2t.net/addgene:20992 ; RRID:Addgene_20992) -
For your References section:
HOXB6 protein is bound to CREB-binding protein and represses globin expression in a DNA binding-dependent, PBX interaction-independent process. Shen W, Chrobak D, Krishnan K, Lawrence HJ, Largman C. J Biol Chem. 2004 Sep 17. 279(38):39895-904. 10.1074/jbc.M404132200 PubMed 15269212