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Addgene

pPBpuro-miRFP703-eDHFR(69K6)-cRaf
(Plasmid #209919)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209919 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPBpuro
  • Backbone size w/o insert (bp) 6886
  • Total vector size (bp) 10466
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miRFP703-eDHFR(69K6)-cRaf
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3510
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Shinya Tsukiji (Addgene plasmid # 172102)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBpuro-miRFP703-eDHFR(69K6)-cRaf was a gift from Michiyuki Matsuda (Addgene plasmid # 209919 ; http://n2t.net/addgene:209919 ; RRID:Addgene_209919)

  • For your References section:

    Low-affinity ligands of the epidermal growth factor receptor are long-range signal transmitters in collective cell migration of epithelial cells. Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K. Cell Rep. 2024 Nov 14;43(11):114986. doi: 10.1016/j.celrep.2024.114986. 10.1016/j.celrep.2024.114986 PubMed 39546398
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