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Addgene

pCSIIbsr-Eevee-ROCK-NES
(Plasmid #209918)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209918 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCSIIbsr
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 12442
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Eevee-ROCK-NES
  • Species
    Synthetic
  • Insert Size (bp)
    2316
  • Tags / Fusion Proteins
    • YPet
    • ECFP

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Tsuyoshi Hirashima (Hino N, Rossetti L, Marín-Llauradó A, Aoki K, Trepat X, Matsuda M, Hirashima T. ERK-Mediated Mechanochemical Waves Direct Collective Cell Polarization. Dev Cell. 2020 Jun 22;53(6):646-660.e8. doi: 10.1016/j.devcel.2020.05.011. Epub 2020 Jun 3. PMID: 32497487.)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSIIbsr-Eevee-ROCK-NES was a gift from Michiyuki Matsuda (Addgene plasmid # 209918 ; http://n2t.net/addgene:209918 ; RRID:Addgene_209918)
  • For your References section:

    Low-affinity ligands of the epidermal growth factor receptor are long-range signal transmitters in collective cell migration of epithelial cells. Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K. Cell Rep. 2024 Nov 14;43(11):114986. doi: 10.1016/j.celrep.2024.114986. 10.1016/j.celrep.2024.114986 PubMed 39546398