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Addgene

HOXB4-FLAG-pSP65
(Plasmid #20990)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP65 (?)
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    in vitro trans/trans

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1237
  • Mutation
    Missing first 14 amino acids
  • Entrez Gene
    HOXB4 (a.k.a. HOX-2.6, HOX2, HOX2F)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (blunted) (destroyed during cloning)
  • 3′ cloning site HindIII (blunted) (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer M13_rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ching Pin Chang

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Though Addgene could confirm the presence of the insert, we could not confirm the vector. Addgene suggests that you sequence this primer if you order it.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB4-FLAG-pSP65 was a gift from Corey Largman (Addgene plasmid # 20990 ; http://n2t.net/addgene:20990 ; RRID:Addgene_20990)

  • For your References section:

    Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins. Chang CP, Shen WF, Rozenfeld S, Lawrence HJ, Largman C, Cleary ML. Genes Dev. 1995 Mar 15. 9(6):663-74. 10.1101/gad.9.6.663 PubMed 7729685
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