pCSIIpuro-TGFa-ScNeo
(Plasmid
#209895)
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PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSIIpuro
- Backbone size w/o insert (bp) 10350
- Total vector size (bp) 12301
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTGFa-ScNeo
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SpeciesH. sapiens (human)
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Insert Size (bp)1947
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Entrez GeneTGFA (a.k.a. TFGA)
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Tags
/ Fusion Proteins
- mScarlet
- HA tag
- GGGSGGGS linker
- mNeonGreen
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSIIpuro-TGFa-ScNeo was a gift from Michiyuki Matsuda (Addgene plasmid # 209895 ; http://n2t.net/addgene:209895 ; RRID:Addgene_209895) -
For your References section:
Low-affinity ligands of the epidermal growth factor receptor are long-range signal transmitters in collective cell migration of epithelial cells. Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K. Cell Rep. 2024 Nov 14;43(11):114986. doi: 10.1016/j.celrep.2024.114986. 10.1016/j.celrep.2024.114986 PubMed 39546398