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GOSR2(G144W)-mCherry
(Plasmid #209887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GOSR2
  • Alt name
    GS27
  • Alt name
    Membrin
  • Alt name
    Golgi SNAP receptor complex member 2
  • Species
    H. sapiens (human)
  • Mutation
    Changed glycine 144 to tryptophan; this mutation causes North Sea progressive myoclonus epilepsy (NSPME)
  • GenBank ID
    NM_001012511.2
  • Entrez Gene
    GOSR2 (a.k.a. Bos1, EPM6, GS27, MYOS)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAACAACTCCGCCCCATT
  • 3′ sequencing primer CACCTTGAAGCGCATGAACT
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned from wildtype GOSR2-mCherry construct

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GOSR2(G144W)-mCherry was a gift from Geert van den Bogaart (Addgene plasmid # 209887 ; http://n2t.net/addgene:209887 ; RRID:Addgene_209887)
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