GOSR1-mCherry
(Plasmid
#209884)
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PurposeExpresses human GOSR1 (GS28) fused to mChery in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209884 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGOSR1
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Alt nameGS28
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Alt nameGOS-28
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Alt nameGolgi SNAP receptor complex member 1
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GenBank IDNP_001007025.1NM_001007024.1
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAACAACTCCGCCCCATT
- 3′ sequencing primer CACCTTGAAGCGCATGAACT (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthetic gene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GOSR1-mCherry was a gift from Geert van den Bogaart (Addgene plasmid # 209884 ; http://n2t.net/addgene:209884 ; RRID:Addgene_209884)