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Addgene

HOXB4-NA-MSCV
(Plasmid #20987)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20987 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Mutation
    N51A (makes DNA binding mutant protein)
  • Entrez Gene
    HOXB4 (a.k.a. HOX-2.6, HOX2, HOX2F)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer MSCV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A possible N->A mutation was deemed acceptable due to its location on the homeodomain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB4-NA-MSCV was a gift from Corey Largman (Addgene plasmid # 20987 ; http://n2t.net/addgene:20987 ; RRID:Addgene_20987)

  • For your References section:

    Cellular proliferation and transformation induced by HOXB4 and HOXB3 proteins involves cooperation with PBX1. Krosl J, Baban S, Krosl G, Rozenfeld S, Largman C, Sauvageau G. Oncogene. 1998 Jul 2. 16(26):3403-12. 10.1038/sj.onc.1201883 PubMed 9692548
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