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Addgene

str-KDEL_Stx5L(M55V)-SBP-mCitrine
(Plasmid #209846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES_neo3
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STX5
  • Alt name
    Syntaxin 5 (long isoform)
  • Species
    H. sapiens (human)
  • Mutation
    Changed Methionine 55 to Valine, causes loss of Stx5S expression.
  • GenBank ID
    NM_003164.5
  • Entrez Gene
    STX5 (a.k.a. CDG2AA, SED5, STX5A)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Streptavidin Binding Protein (SBP) (C terminal on insert)
    • mCitrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site Sma1 (not destroyed)
  • 5′ sequencing primer IRES-F
  • 3′ sequencing primer pEGFP-all
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    str-KDEL_Stx5L(M55V)-SBP-mCitrine was a gift from Geert van den Bogaart (Addgene plasmid # 209846 ; http://n2t.net/addgene:209846 ; RRID:Addgene_209846)

  • For your References section:

    Congenital disorder of glycosylation caused by starting site-specific variant in syntaxin-5. Linders PTA, Gerretsen ECF, Ashikov A, Vals MA, de Boer R, Revelo NH, Arts R, Baerenfaenger M, Zijlstra F, Huijben K, Raymond K, Muru K, Fjodorova O, Pajusalu S, Ounap K, Ter Beest M, Lefeber D, van den Bogaart G. Nat Commun. 2021 Oct 28;12(1):6227. doi: 10.1038/s41467-021-26534-y. 10.1038/s41467-021-26534-y PubMed 34711829
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