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pHAND1-T2A-dTom-PGK-Neo
(Plasmid #209838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209838 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 3522
  • Total vector size (bp) 6935
  • Vector type
    Human Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HAND1-T2A-dTomato-PGK-Neo-HAND1
  • Alt name
    Heart and neural crest derivatives expressed 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4237
  • GenBank ID
    NM_004821 NM_004821
  • Entrez Gene
    HAND1 (a.k.a. Hxt, Thing1, bHLHa27, eHand)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer GTCATAGCTGTTTCCTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAND1-T2A-dTom-PGK-Neo was a gift from Matthew Birket (Addgene plasmid # 209838 ; http://n2t.net/addgene:209838 ; RRID:Addgene_209838)

  • For your References section:

    The generation and validation of a dual cardiac HAND1-Tomato NKX2-5-GFP human embryonic stem cell line UMANe002-A-3. Lynch AT, Douglas M, Kimber SJ, Birket MJ. Stem Cell Res. 2024 Mar 4;77:103342. doi: 10.1016/j.scr.2024.103342. 10.1016/j.scr.2024.103342 PubMed 38460234
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