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Addgene

LARP1_323-509
(Plasmid #209800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LARP1 La-module
  • Species
    H. sapiens (human)
  • Mutation
    aa 323-509 only
  • Entrez Gene
    LARP1 (a.k.a. LARP, Lar1, Lhp1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer T7 Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LARP1_323-509 was a gift from Kalle Gehring (Addgene plasmid # 209800 ; http://n2t.net/addgene:209800 ; RRID:Addgene_209800)

  • For your References section:

    Structural basis of 3'-end poly(A) RNA recognition by LARP1. Kozlov G, Mattijssen S, Jiang J, Nyandwi S, Sprules T, Iben JR, Coon SL, Gaidamakov S, Noronha AM, Wilds CJ, Maraia RJ, Gehring K. Nucleic Acids Res. 2022 Sep 9;50(16):9534-9547. doi: 10.1093/nar/gkac696. 10.1093/nar/gkac696 PubMed 35979957
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