hPRL3_1-169
(Plasmid #209796)

• Purpose: Bacterial expression of human PRL-3 without farnesylation motif
• Depositing Lab: Kalle Gehring
• Publication: Kozlov et al J Biol Chem. 2004 Mar 19. 279(12):11882-9.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5708
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRL-3
• Species: H. sapiens (human)
• Mutation: aa 1-169 only
• Entrez Gene: PTP4A3 (a.k.a. PRL-3, PRL-R, PRL3)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: T7 Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry