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Addgene

hPRL2_1-163
(Plasmid #209794)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209794 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRL-2
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-163 only
  • Entrez Gene
    PTP4A2 (a.k.a. HH13, HH7-2, HU-PP-1, OV-1, PRL-2, PRL2, PTP4A, PTPCAAX2, ptp-IV1a, ptp-IV1b)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer T7 Rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    codon-optimized gene synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPRL2_1-163 was a gift from Kalle Gehring (Addgene plasmid # 209794 ; http://n2t.net/addgene:209794 ; RRID:Addgene_209794)

  • For your References section:

    PRL3 pseudophosphatase activity is necessary and sufficient to promote metastatic growth. Kozlov G, Funato Y, Chen YS, Zhang Z, Illes K, Miki H, Gehring K. J Biol Chem. 2020 Aug 14;295(33):11682-11692. doi: 10.1074/jbc.RA120.014464. Epub 2020 Jun 22. 10.1074/jbc.RA120.014464 PubMed 32571875
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