HOXA9-pRSET
(Plasmid
#20979)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20979 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSET-c
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXA9
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SpeciesH. sapiens (human)
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Insert Size (bp)900
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Entrez GeneHOXA9 (a.k.a. ABD-B, HOX1, HOX1.7, HOX1G)
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- Xpress (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer Xpress-fwd
- 3′ sequencing primer T7-terminal (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGuy Sauvageau
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cDNA cloned from human bone marrow cells. Addgene has not been able to confirm the Flag tag.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXA9-pRSET was a gift from Corey Largman (Addgene plasmid # 20979 ; http://n2t.net/addgene:20979 ; RRID:Addgene_20979)
Map uploaded by the depositor.
