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Addgene

PABPC1_544-636_pGEX6P1
(Plasmid #209789)

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Item Catalog # Description Quantity Price (USD)
Plasmid 209789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4984
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mlle domain of PABPC1
  • Alt name
    PABC domain of PABPC1
  • Species
    H. sapiens (human)
  • Mutation
    aa 544-636 only
  • Entrez Gene
    PABPC1 (a.k.a. PAB1, PABP, PABP1, PABPC2, PABPL1)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX Fwd
  • 3′ sequencing primer pGEX Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PABPC1_544-636_pGEX6P1 was a gift from Kalle Gehring (Addgene plasmid # 209789 ; http://n2t.net/addgene:209789 ; RRID:Addgene_209789)

  • For your References section:

    Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. Kozlov G, De Crescenzo G, Lim NS, Siddiqui N, Fantus D, Kahvejian A, Trempe JF, Elias D, Ekiel I, Sonenberg N, O'Connor-McCourt M, Gehring K. EMBO J. 2004 Jan 28;23(2):272-81. doi: 10.1038/sj.emboj.7600048. Epub 2003 Dec 18. 10.1038/sj.emboj.7600048 PubMed 14685257
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