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pAAV-CASI-InteinC-SpRY C aa714-1368-U6-Camk2d sgRNA
(Plasmid #209788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold
  • Backbone size w/o insert (bp) 7245
  • Total vector size (bp) 7246
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpRY C, Camk2d sgRNA
  • Alt name
    Split-SpRY C-terminal half and Camk2d sgRNA
  • gRNA/shRNA sequence
    TCCTGCCTGTGCATCATGGA
  • Species
    Synthetic
  • Promoter CASI

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CASI-InteinC-SpRY C aa714-1368-U6-Camk2d sgRNA was a gift from Yuxuan Guo (Addgene plasmid # 209788 ; http://n2t.net/addgene:209788 ; RRID:Addgene_209788)

  • For your References section:

    MicroRNA-122-Mediated Liver Detargeting Enhances the Tissue Specificity of Cardiac Genome Editing. Yang L, Liu Z, Chen G, Chen Z, Guo C, Ji X, Cui Q, Sun Y, Hu X, Zheng Y, Li Y, Gao F, Chen L, Zhou P, Pu WT, Guo Y. Circulation. 2024 May 28;149(22):1778-1781. doi: 10.1161/CIRCULATIONAHA.123.065438. Epub 2024 May 28. 10.1161/CIRCULATIONAHA.123.065438 PubMed 38805581
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