AAV-cTNT-Cre
(Plasmid #209785)

• Purpose: Expresses Cre by the specific cTNT promoter
• Depositing Lab: Yuxuan Guo
• Publication: Yang et al Circulation. 2024 May 28;149(22):1778-1781. doi: 10.1161/CIRCULATIONAHA.123.065438. Epub 2024 May 28.

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Backbone

• Vector backbone: 2xU6gRNA-TNT-Cre
• Backbone size w/o insert (bp): 5958
• Total vector size (bp): 5237
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cre
• Alt name: Cre
• Species: Synthetic
• Insert Size (bp): 1056
• Promoter: cTNT

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gggaaacgcctggtatcttt
• 3′ sequencing primer: tctattgggaaccaagctgg

Resource Information

• Supplemental Documents:
  • PD339-aav-ctnt-crE.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.06.29.546982v1 for bioRxiv preprint.