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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS
(Plasmid #209782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV-U6-msCAMK2d-SgRNA-scaffold-cTNT-SaCas9-HA-OLLAS
  • Backbone size w/o insert (bp) 7500
  • Total vector size (bp) 7499
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA
  • Alt name
    sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    21
  • Promoter cTNT
  • Tags / Fusion Proteins
    • HA (C terminal on backbone)
    • OLLAS (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cttgaaagtatttcgatttcttggc
  • 3′ sequencing primer cctctttgaacagccgcac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS was a gift from Yuxuan Guo (Addgene plasmid # 209782 ; http://n2t.net/addgene:209782 ; RRID:Addgene_209782)

  • For your References section:

    MicroRNA-122-Mediated Liver Detargeting Enhances the Tissue Specificity of Cardiac Genome Editing. Yang L, Liu Z, Chen G, Chen Z, Guo C, Ji X, Cui Q, Sun Y, Hu X, Zheng Y, Li Y, Gao F, Chen L, Zhou P, Pu WT, Guo Y. Circulation. 2024 May 28;149(22):1778-1781. doi: 10.1161/CIRCULATIONAHA.123.065438. Epub 2024 May 28. 10.1161/CIRCULATIONAHA.123.065438 PubMed 38805581
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