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Addgene

Human HOXA9-MSCV-short
(Plasmid #20978)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20978 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Mutation
    Missing amino acid #1. Missing 3'UTR. Coding sequence only.
  • Entrez Gene
    HOXA9 (a.k.a. ABD-B, HOX1, HOX1.7, HOX1G)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN-5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IRES-GFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human HOXA9-MSCV-short was a gift from Corey Largman (Addgene plasmid # 20978 ; http://n2t.net/addgene:20978 ; RRID:Addgene_20978)

  • For your References section:

    Protein kinase C-mediated phosphorylation of the leukemia-associated HOXA9 protein impairs its DNA binding ability and induces myeloid differentiation. Vijapurkar U, Fischbach N, Shen W, Brandts C, Stokoe D, Lawrence HJ, Largman C. Mol Cell Biol. 2004 May . 24(9):3827-37. 10.1128/MCB.24.9.3827-3837.2004 PubMed 15082777
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