pPB-CAG-NPHP1
(Plasmid
#209769)
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PurposeHuman NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPB-PGK-destination
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Backbone manufacturerAustin Smith
- Backbone size w/o insert (bp) 7725
- Total vector size (bp) 10101
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Vector typeMammalian Expression ; PiggyBac Transposon
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNPHP1
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Alt nameNephrocystin 1
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1884
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GenBank IDNM_001128178.3
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Entrez GeneNPHP1 (a.k.a. JBTS4, NPH1, SLSN1)
- Promoter CAG
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Tag
/ Fusion Protein
- HA tag (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAGGS-5
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CAG-NPHP1 was a gift from Yohei Hayashi (Addgene plasmid # 209769 ; http://n2t.net/addgene:209769 ; RRID:Addgene_209769)