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Addgene

pPB-CAG-NPHP1
(Plasmid #209769)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209769 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPB-PGK-destination
  • Backbone manufacturer
    Austin Smith
  • Backbone size w/o insert (bp) 7725
  • Total vector size (bp) 10101
  • Vector type
    Mammalian Expression ; PiggyBac Transposon
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPHP1
  • Alt name
    Nephrocystin 1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1884
  • GenBank ID
    NM_001128178.3
  • Entrez Gene
    NPHP1 (a.k.a. JBTS4, NPH1, SLSN1)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-CAG-NPHP1 was a gift from Yohei Hayashi (Addgene plasmid # 209769 ; http://n2t.net/addgene:209769 ; RRID:Addgene_209769)