pPB-CAG-Venus-Akaluc
(Plasmid #209768)

• Purpose: Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).
• Depositing Lab: Yohei Hayashi
• Publication: PiggyBac transposon vectors (unpublished)

Backbone

• Vector backbone: pPB-PGK-destination
• Backbone manufacturer: Austin Smith
• Backbone size w/o insert (bp): 7725
• Total vector size (bp): 10101
• Vector type: Mammalian Expression, Luciferase ; PiggyBac Transposon
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Venus-Akaluc
• Species: Synthetic
• Insert Size (bp): 2376
• Promoter: CAG

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pCAGGS-5
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pPB-CAG-Venus-Akaluc.dna
• A portion of this plasmid was derived from a plasmid made by: Akaluc is cloned from pcDNA3 Venus-Akaluc. The pcDNA3 Venus-Akaluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15781).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://dnaconda.riken.jp/search/RDB_clone/RDB15/RDB15781.html