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Addgene

rEstus
(Plasmid #209767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)_Puro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rEstus
  • Alt name
    ASAP3-N138G-Y141T-S318A-Q396R
  • Species
    G. gallus (chicken); A.victoria
  • Insert Size (bp)
    1281
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG
  • 3′ sequencing primer TAG AAG GCA CAG TCG AGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.05.560122 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rEstus was a gift from Stefan Heinemann (Addgene plasmid # 209767 ; http://n2t.net/addgene:209767 ; RRID:Addgene_209767)

  • For your References section:

    An ultrasensitive voltage indicator uncovers the electrical activity of non-excitable cells. Rühl P., Nair, A. G., Gawande, N., Dehiwalage, S. N. C. W., Münster, L., Schönherr, R., & Heinemann, S. H.. bioRxiv 2023. 10.1101/2023.10.05.560122
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