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Addgene

pAMC042z21
(Plasmid #209747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209747 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RepA(rc)(N99D) SC101 T0-BS7 araC(rc) pBAD SD8 ProK 5 tRNA operon tetA-orfL bla(rc)
  • Backbone manufacturer
    self
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sfGFP - tRNA operon
  • Species
    Synthetic
  • Insert Size (bp)
    1713
  • Mutation
    sfGFP - Valine 93 AGGA quadruplet codon, Tyrosine 151 UAGA quadruplet codon

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.15.594368 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAMC042z21 was a gift from Ahmed Badran (Addgene plasmid # 209747 ; http://n2t.net/addgene:209747 ; RRID:Addgene_209747)

  • For your References section:

    Efficient genetic code expansion without host genome modifications. Costello A, Peterson AA, Lanster DL, Li Z, Carver GD, Badran AH. Nat Biotechnol. 2024 Sep 11. doi: 10.1038/s41587-024-02385-y. 10.1038/s41587-024-02385-y PubMed 39261591
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