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Addgene

pAMC070n1a12
(Plasmid #209746)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209746 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAB228
  • Backbone manufacturer
    self
  • Backbone size w/o insert (bp) 1666
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Trimethoprim
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS
  • Species
    Synthetic
  • Insert Size (bp)
    5114

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.15.594368 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAMC070n1a12 was a gift from Ahmed Badran (Addgene plasmid # 209746 ; http://n2t.net/addgene:209746 ; RRID:Addgene_209746)
  • For your References section:

    Efficient genetic code expansion without host genome modifications. Costello A, Peterson AA, Lanster DL, Li Z, Carver GD, Badran AH. Nat Biotechnol. 2024 Sep 11. doi: 10.1038/s41587-024-02385-y. 10.1038/s41587-024-02385-y PubMed 39261591