pAB228p
(Plasmid
#209742)
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PurposeColA BBa_B0014 tetAp SD8 MmPylRS-[wt] T3Te-T7Te DHFR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209742 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAB228
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Backbone manufacturerself
- Backbone size w/o insert (bp) 1666
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Trimethoprim
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMmPylRS
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SpeciesSynthetic
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Insert Size (bp)1365
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.05.15.594368 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAB228p was a gift from Ahmed Badran (Addgene plasmid # 209742 ; http://n2t.net/addgene:209742 ; RRID:Addgene_209742) -
For your References section:
Efficient genetic code expansion without host genome modifications. Costello A, Peterson AA, Lanster DL, Li Z, Carver GD, Badran AH. Nat Biotechnol. 2024 Sep 11. doi: 10.1038/s41587-024-02385-y. 10.1038/s41587-024-02385-y PubMed 39261591