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Addgene

HOXA9-pGEX
(Plasmid #20974)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-3
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA9
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    950
  • Entrez Gene
    Hoxa9 (a.k.a. D6a9, Hox-1.7)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI/BalI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA9-pGEX was a gift from Corey Largman (Addgene plasmid # 20974 ; http://n2t.net/addgene:20974 ; RRID:Addgene_20974)

  • For your References section:

    HOXA9 forms triple complexes with PBX2 and MEIS1 in myeloid cells. Shen WF, Rozenfeld S, Kwong A, Koem ves LG, Lawrence HJ, Largman C. Mol Cell Biol. 1999 Apr . 19(4):3051-61. PubMed 10082572
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