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Addgene

ataxin3:1-431_Q79_BV
(Plasmid #209733)

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Item Catalog # Description Quantity Price (USD)
Plasmid 209733 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-SBP-TEV-LIC
  • Backbone manufacturer
    SGC, pFBOH-MHL modified
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ataxin 3
  • Alt name
    ataxin3:TOC031-F04:C247559
  • Species
    H. sapiens (human)
  • Entrez Gene
    ATXN3 (a.k.a. AT3, ATX3, JOS, MJD, MJD1, SCA3)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6xHis-SBP-TEV (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ataxin3:1-431_Q79_BV was a gift from Cheryl Arrowsmith (Addgene plasmid # 209733 ; http://n2t.net/addgene:209733 ; RRID:Addgene_209733)
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