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Addgene

Epsin1 deltaUIM-ECFP
(Plasmid #20973)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20973 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pECFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Epsin1 deltaUIM
  • Alt name
    Epsin1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1475
  • GenBank ID
    NC_005100
  • Entrez Gene
    Epn1
  • Tag / Fusion Protein
    • ECFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site AgeI (unknown if destroyed)
  • 5′ sequencing primer CMV-Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gift of P. De Camilli (Yale University, New Haven, CT) for providing plasmids containing rat epsin 1 cDNA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Epsin1 deltaUIM-ECFP was a gift from Xiaowei Zhuang (Addgene plasmid # 20973 ; http://n2t.net/addgene:20973 ; RRID:Addgene_20973)
  • For your References section:

    Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Chen C, Zhuang X. Proc Natl Acad Sci U S A. 2008 Aug 19. 105(33):11790-5. 10.1073/pnas.0803711105 PubMed 18689690