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plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
(Plasmid #209728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    plenti.CamKII
  • Total vector size (bp) 10875
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    (cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
  • Insert Size (bp)
    1629
  • Promoter CamKII

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CamKII-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3 was a gift from Timothy Ryan (Addgene plasmid # 209728 ; http://n2t.net/addgene:209728 ; RRID:Addgene_209728)

  • For your References section:

    iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP. Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown TA, Ryan TA. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 10.1073/pnas.2314604121. Epub 2024 May 15. 10.1073/pnas.2314604121 PubMed 38748581
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