pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag
(Plasmid #209722)

• Purpose: Expresses mitochondrially targeted low affinity ratiometric ATP sensor
• Depositing Lab: Timothy Ryan
• Publication: Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 10.1073/pnas.2314604121. Epub 2024 May 15.

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Backbone

• Vector backbone: pAAV.CAG
• Total vector size (bp): 7201
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: (mito).iATPSnFR2.A95A.A119L.HaloTag
• Insert Size (bp): 2490
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: CAG-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.08.24.554624v1 for bioRxiv preprint.