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Addgene

pCMV-NLS-MBP-rATOM(WDR5)
(Plasmid #209710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-MBP-rATOM(WDR5)
  • Species
    Synthetic
  • Tag / Fusion Protein
    • NLS-MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.28.534597 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-NLS-MBP-rATOM(WDR5) was a gift from Stewart Loh (Addgene plasmid # 209710 ; http://n2t.net/addgene:209710 ; RRID:Addgene_209710)

  • For your References section:

    Adaptable, turn-on maturation (ATOM) fluorescent biosensors for multiplexed detection in cells. Sekhon H, Ha JH, Presti MF, Procopio SB, Jarvis AR, Mirsky PO, John AM, Loh SN. Nat Methods. 2023 Nov 9. doi: 10.1038/s41592-023-02065-w. 10.1038/s41592-023-02065-w PubMed 37945909
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