pAAV-Dlx-FLEX-hM4Di-mCherry
(Plasmid #209701)

• Purpose: Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner
• Depositing Lab: Peer Wulff
• Publication: Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-1. doi: 10.1016/j.neuron.2024.03.019.

Backbone

• Vector backbone: pAAV
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hM4D(Gi)-mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Eco72I (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: gcgtaaaaggagcaacata
• 3′ sequencing primer: CTACGTCAACAGCACCATC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mDlx enhancer from Addgene_83900 and hM4D(Gi)-mCherry from Addgene_50475

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry