pAAV-Dlx-FLEX-hM4Di-mCherry
(Plasmid
#209701)
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PurposeExpresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209701 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehM4D(Gi)-mCherry
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eco72I (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer gcgtaaaaggagcaacata
- 3′ sequencing primer CTACGTCAACAGCACCATC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymDlx enhancer from Addgene_83900 and hM4D(Gi)-mCherry from Addgene_50475
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Dlx-FLEX-hM4Di-mCherry was a gift from Peer Wulff (Addgene plasmid # 209701 ; http://n2t.net/addgene:209701 ; RRID:Addgene_209701) -
For your References section:
Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning. Rangel Guerrero DK, Balueva K, Barayeu U, Baracskay P, Gridchyn I, Nardin M, Roth CN, Wulff P, Csicsvari J. Neuron. 2024 Apr 10:S0896-6273(24)00197-1. doi: 10.1016/j.neuron.2024.03.019. 10.1016/j.neuron.2024.03.019 PubMed 38636524