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MAC3_C_CCDC22(T17A)
(Plasmid #209694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MAC3-tag_C (Plasmid #185481)
  • Backbone manufacturer
    Markku Varjosalo lab
  • Backbone size w/o insert (bp) 5876
  • Total vector size (bp) 7758
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCDC22
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1881
  • Mutation
    T17A, changed Threonine 17 to Alanine
  • GenBank ID
    NM_014008
  • Entrez Gene
    CCDC22 (a.k.a. CXorf37, JM1, RTSC2)
  • Tag / Fusion Protein
    • MAC3-tag (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TGT AAA ACG ACG GCC AGT
  • 3′ sequencing primer CAG GAA ACA GCT ATG ACC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human ORFeome collection

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.03.535349 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC3_C_CCDC22(T17A) was a gift from Markku Varjosalo (Addgene plasmid # 209694 ; http://n2t.net/addgene:209694 ; RRID:Addgene_209694)

  • For your References section:

    Structure and interactions of the endogenous human Commander complex. Laulumaa S, Kumpula EP, Huiskonen JT, Varjosalo M. Nat Struct Mol Biol. 2024 Jun;31(6):925-938. doi: 10.1038/s41594-024-01246-1. Epub 2024 Mar 8. 10.1038/s41594-024-01246-1 PubMed 38459129
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