MAC_N_CCDC93
(Plasmid #209687)

• Purpose: Expresses MAC-tagged CCDC93 in mammalian cells
• Depositing Lab: Markku Varjosalo
• Publication: Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-938. doi: 10.1038/s41594-024-01246-1. Epub 2024 Mar 8.

Backbone

• Vector backbone: MAC-tag-N (#108078)
• Backbone manufacturer: Markku Varjosalo lab
• Backbone size w/o insert (bp): 6312
• Total vector size (bp): 8217
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CCDC93
• Species: H. sapiens (human)
• Insert Size (bp): 1905
• GenBank ID: NM_019044
• Entrez Gene: CCDC93
• Tag / Fusion Protein:
  • MAC-tag (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: TGT AAA ACG ACG GCC AGT
• 3′ sequencing primer: CAG GAA ACA GCT ATG ACC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human ORFeome collection

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.03.535349 for bioRxiv preprint.