MAC_N_COMMD1
(Plasmid
#209674)
-
PurposeExpresses MAC-tagged COMMD1 in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 209674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneMAC-tag-N (#108078)
-
Backbone manufacturerMarkku Varjosalo lab
- Backbone size w/o insert (bp) 6312
- Total vector size (bp) 6882
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCOMMD1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)570
-
GenBank IDNM_152516
-
Entrez GeneCOMMD1 (a.k.a. C2orf5, MURR1)
-
Tag
/ Fusion Protein
- MAC-tag (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 uni (-21)
- 3′ sequencing primer M13 rev (-29) (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byHuman ORFeome collection
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.04.03.535349 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MAC_N_COMMD1 was a gift from Markku Varjosalo (Addgene plasmid # 209674 ; http://n2t.net/addgene:209674 ; RRID:Addgene_209674) -
For your References section:
Structure and interactions of the endogenous human Commander complex. Laulumaa S, Kumpula EP, Huiskonen JT, Varjosalo M. Nat Struct Mol Biol. 2024 Jun;31(6):925-938. doi: 10.1038/s41594-024-01246-1. Epub 2024 Mar 8. 10.1038/s41594-024-01246-1 PubMed 38459129