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Addgene

pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag
(Plasmid #209651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iATPSnFR2.S29W.HaloTag
  • Species
    Synthetic
  • Mutation
    S29W.A95K
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.08.24.554624 for bioRxiv preprint.

There is a 149 bp deletion in part of the ITR and upstream of it.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag was a gift from Tim Brown & HHMI-JRC Tool Translation Team (Addgene plasmid # 209651 ; http://n2t.net/addgene:209651 ; RRID:Addgene_209651)

  • For your References section:

    iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP. Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown TA, Ryan TA. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 10.1073/pnas.2314604121. Epub 2024 May 15. 10.1073/pnas.2314604121 PubMed 38748581
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