pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro
(Plasmid
#209642)
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PurposeTo install m.G3635 mutation on mtDNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209642 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameL17-FZY2-S100N
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro was a gift from Bin Shen (Addgene plasmid # 209642 ; http://n2t.net/addgene:209642 ; RRID:Addgene_209642) -
For your References section:
Developing mitochondrial base editors with diverse context compatibility and high fidelity via saturated spacer library. Sun H, Wang Z, Shen L, Feng Y, Han L, Qian X, Meng R, Ji K, Liang D, Zhou F, Lou X, Zhang J, Shen B. Nat Commun. 2023 Oct 19;14(1):6625. doi: 10.1038/s41467-023-42359-3. 10.1038/s41467-023-42359-3 PubMed 37857619