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pPicZ:mTREK-1_cryst_I110D
(Plasmid #209640)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209640 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPicZ
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
  • Species
    M. musculus (mouse)
  • Mutation
    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A92P, N95S, S96D, T97Q, N119A, S300A, E306A, I110D
  • Tag / Fusion Protein
    • SNS- 3C_cleavage_site-TAAA-GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer AOX5
  • 3′ sequencing primer AOX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPicZ:mTREK-1_cryst_I110D was a gift from Dan Minor (Addgene plasmid # 209640 ; http://n2t.net/addgene:209640 ; RRID:Addgene_209640)

  • For your References section:

    Polynuclear Ruthenium Amines Inhibit K(2P) Channels via a "Finger in the Dam" Mechanism. Pope L, Lolicato M, Minor DL Jr. Cell Chem Biol. 2020 May 21;27(5):511-524.e4. doi: 10.1016/j.chembiol.2020.01.011. Epub 2020 Feb 13. 10.1016/j.chembiol.2020.01.011 PubMed 32059793
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