Skip to main content
Addgene

GFP-mId1
(Plasmid #20963)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Inhibitor of DNA binding 1
  • Alt name
    Id1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500
  • GenBank ID
    NM_010495
  • Entrez Gene
    Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-mId1 was a gift from Robert Benezra (Addgene plasmid # 20963 ; http://n2t.net/addgene:20963 ; RRID:Addgene_20963)

  • For your References section:

    High levels of Id1 expression define B1 type adult neural stem cells. Nam HS, Benezra R. Cell Stem Cell. 2009 Nov 6. 5(5):515-26. 10.1016/j.stem.2009.08.017 PubMed 19896442
Related items:
Commonly requested with: