pCAGIG-FLAG-SHTN1LRRR>GGG
(Plasmid
#209570)
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PurposeMammalian expression of Shtn1 long isoform with RG mutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209570 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGIG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameShtn1 long isoform with RG mutation
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Alt nameShootin1; mKIAA1598; 4930506M07Rik
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SpeciesM. musculus (mouse)
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MutationRRR to GGG
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Entrez Gene4930506M07Rik (a.k.a. KIAA1598, Kiaa1598, Shootin1, mKIAA1598)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGIG-FLAG-SHTN1LRRR>GGG was a gift from Sika Zheng (Addgene plasmid # 209570 ; http://n2t.net/addgene:209570 ; RRID:Addgene_209570) -
For your References section:
Axonogenesis Is Coordinated by Neuron-Specific Alternative Splicing Programming and Splicing Regulator PTBP2. Zhang M, Ergin V, Lin L, Stork C, Chen L, Zheng S. Neuron. 2019 Feb 20;101(4):690-706.e10. doi: 10.1016/j.neuron.2019.01.022. Epub 2019 Feb 4. 10.1016/j.neuron.2019.01.022 PubMed 30733148