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Addgene

pFPV-mCherry
(Plasmid #20956)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFPV
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Discosoma
  • Insert Size (bp)
    711
  • GenBank ID
    AY678264

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mCherry from Roger Tsien
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Shaner et al., (2004)
Nature Biotechnology 22,1567-1572

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFPV-mCherry was a gift from Olivia Steele-Mortimer (Addgene plasmid # 20956 ; http://n2t.net/addgene:20956 ; RRID:Addgene_20956)

  • For your References section:

    Dynamic behavior of Salmonella-induced membrane tubules in epithelial cells. Drecktrah D, Levine-Wilkinson S, Dam T, Winfree S, Knodler LA, Schroer TA, Steele-Mortimer O. Traffic. 2008 Dec . 9(12):2117-29. 10.1111/j.1600-0854.2008.00830.x PubMed 18785994
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