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Addgene

pBTK1061
(Plasmid #209550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK095
  • Backbone size w/o insert (bp) 1887
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
  • Species
    Synthetic
  • Insert Size (bp)
    3853

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI-v2 (destroyed during cloning)
  • 3′ cloning site BsaI-v2 (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.19.558440 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTK1061 was a gift from Jeffrey Barrick (Addgene plasmid # 209550 ; http://n2t.net/addgene:209550 ; RRID:Addgene_209550)

  • For your References section:

    Single-step genome engineering in the bee gut symbiont Snodgrassella alvi. Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, Barrick JE. bioRxiv 2023.09.19.558440 10.1101/2023.09.19.558440
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