pcDNA3.1/VChR1-EYFP
(Plasmid #20955)

• Purpose: Mammalian expression of VChR1 fused to EYFP, for neuronal excitation
• Depositing Lab: Karl Deisseroth
• Publication: Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5545
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: volvox channelrhodopsin-1
• Alt name: VChR1
• Species: V. carteri
• Insert Size (bp): 1626
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry