pBTK1054
(Plasmid
#209544)
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PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome at a neutral site
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 209544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYTK095
- Backbone size w/o insert (bp) 1886
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRecombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
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SpeciesSynthetic
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Insert Size (bp)3959
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI-v2 (destroyed during cloning)
- 3′ cloning site BsaI-v2 (destroyed during cloning)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.09.19.558440 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBTK1054 was a gift from Jeffrey Barrick (Addgene plasmid # 209544 ; http://n2t.net/addgene:209544 ; RRID:Addgene_209544) -
For your References section:
Single-step genome engineering in the bee gut symbiont Snodgrassella alvi. Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, Barrick JE. bioRxiv 2023.09.19.558440 10.1101/2023.09.19.558440
