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Addgene

pBTK1050
(Plasmid #209540)

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Item Catalog # Description Quantity Price (USD)
Plasmid 209540 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBTK1001
  • Backbone size w/o insert (bp) 1818
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
  • Species
    Snodgrassella alvi wkB2
  • Insert Size (bp)
    1057

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI-v2 (destroyed during cloning)
  • 3′ cloning site BsmBI-v2 (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.19.558440 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTK1050 was a gift from Jeffrey Barrick (Addgene plasmid # 209540 ; http://n2t.net/addgene:209540 ; RRID:Addgene_209540)

  • For your References section:

    Single-step genome engineering in the bee gut symbiont Snodgrassella alvi. Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, Barrick JE. bioRxiv 2023.09.19.558440 10.1101/2023.09.19.558440
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