pLenti-CaMKIIa-VChR1-EYFP-WPRE
(Plasmid #20954)

• Purpose: 3rd gen lentiviral expression of VChR1 fused to EYFP driven by CaMKIIa promoter for optogenetic activation
• Depositing Lab: Karl Deisseroth
• Publication: Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Backbone

• Vector backbone: pLenti-CaMKIIa
• Backbone size w/o insert (bp): 9250
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: volvox channelrhodopsin-1
• Alt name: VChR1
• Species: V. carteri
• Insert Size (bp): 1626
• Promoter: CaMKIIa
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see http://www.optogenetics.org

for additional information.