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Addgene

pGL4.23_Ptgs2
(Plasmid #209519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.23
  • Backbone size w/o insert (bp) 4283
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ptgs2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    801
  • Entrez Gene
    Ptgs2 (a.k.a. COX2, Cox-2, PES-2, PGHS-2, PHS II, PHS-2, Pghs2, TIS10, gripghs)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer AGCATTCCGATGAAGTGGAGCT
  • 3′ sequencing primer GGAGGTGGCAGTAGTGGTGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.23_Ptgs2 was a gift from Jennifer Mitchell (Addgene plasmid # 209519 ; http://n2t.net/addgene:209519 ; RRID:Addgene_209519)

  • For your References section:

    SOX4 exerts contrasting regulatory effects on labor-associated gene promoters in myometrial cells. Khader N, Shchuka VM, Dorogin A, Shynlova O, Mitchell JA. PLoS One. 2024 Apr 18;19(4):e0297847. doi: 10.1371/journal.pone.0297847. eCollection 2024. 10.1371/journal.pone.0297847 PubMed 38635533
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