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Addgene

EC67842
(Plasmid #209516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    CRISPR
  • Selectable markers
    RFP & hygromycin for plant

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Barley GoldenGate binary vector with Cas12a

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC67842 was a gift from Wendy Harwood & Cristobal Uauy (Addgene plasmid # 209516 ; http://n2t.net/addgene:209516 ; RRID:Addgene_209516)

  • For your References section:

    An Optimised CRISPR Cas9 and Cas12a Toolkit for Barley and Wheat. Lawrenson T, Clarke M, Kirby R, Forner M, Steuernagel B, Harwood W. Research Square, 12 March 2024, PREPRINT 10.21203/rs.3.rs-4024107/v1
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